Breeding: The Two Spot Goby are very difficult to maintain in the home aquarium, as most slowly starve to death and should be only kept as a mated pair. The breeding behavior of the Two Spot Goby is very unique. They form monogamous pairs and share a burrow. The gravid female nibbles the male's body before spawning and then enters the burrow and deposits her eggs. After spawning the male is sealed in the burrow at which time he fertilizes the eggs, guards and possibly aerates them. For two to three days the male resides in the burrow.

During this period the female Two Spot Goby will occasionally open the chamber and liberate the male and the pair will engage in burrow maintenance. The male then re-enters the burrow and is once again sealed in. Eventually the male exits the burrow one last time and both parents close the entrance and move to a new burrow or reopen an old one nearby. Finally, the burrow is opened a final time and a single juvenile fish emerges.